RNA Seq of mus musculus:adult female spleen treated with DR3 agonist ... - SRA

SRX5590591: RNA Seq of mus musculus:adult female spleen treated with DR3 agonist (4C12) antibody
1 ILLUMINA (Illumina HiSeq 4000) run: 52.5M spots, 8G bases, 2.9Gb downloads

Design: RNA was extracted using TRIzol method with chloroform phase separation and isopropanol precipitation then applied to the QIAGEN miRNeasy mini (QIAGEN, Cat. #217004) column for RNA purification. All RNAs were checked on a NANODRP1000 and Agilent bioanalyzer 2100 PICO chip for RNA purity and integrity. The KAPA mRNA HyperPrep Kits (KK8580) with the IDT for Illumina Dual Index Adapter kit (Caat. #20021454) per the manufacturers protocol. Briefly, mRNA was captured using magnetic oligo-dT beads. Fragmentation was performed using heat and magnesium. 1st strand cDNA synthesis was completed using random priming. Combined 2nd strand synthesis and A-tailing, adapter ligation, library amplification, and KAPA Pure Beads clean-ups were performed for library preparation. The strand marked with dUTP is not amplified, allowing strand-specific sequencing. All final libraries were checked on Agilents bioanalyzer 2100 High Sensitivity DNA Chip. An equal amount of cDNA library from each sample was pooled for sequencing on the Illumina HiSeq 4000 platform.

Submitted by: Stanford University Medical Center

Study: Effect of DR3 agonists on murine Tregs

PRJNA529746 • SRP189821 • All experiments • All runs

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Mice expressing luciferase and GFP under control of the FoxP3 promoter were treated with either the DR3 agonist antibody 4C12 or the DR3 agonist fusion protein TL1A-Ig with low-dose IL-2 (or isotype control antibody), regulatory T cells were sorted from spleens on day 7, and bulk RNA sequencing was performed.

Sample: 4C12 Ab-1 (Experiment 2)

SAMN11282889 • SRS4551762 • All experiments • All runs

Organism: Mus musculus

Library:

Name: MM4-1-1-19

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 52.5M spots, 8G bases, 2.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR8801888	52,524,225	8G	2.9Gb	2019-07-08

ID:: 7541580

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